Blo t 2: Group 2 allergen from the dust mite Blomia tropicalis

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Nuclear magnetic resonance structure and IgE epitopes of Blo t 5, a major dust mite allergen.

A high incidence of sensitization to Blomia tropicalis, the predominant house dust mite species in tropical regions, is strongly associated with allergic diseases in Singapore, Malaysia, and Brazil. IgE binding to the group 5 allergen, Blo t 5, is found to be the most prevalent among all B. tropicalis allergens. The NMR structure of Blo t 5 determined represents a novel helical bundle structure...

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Allergenic cross-reactivity between Blomia tropicalis and Blomia kulagini (Acari: Echymiopodidae) extracts from optimized mite cultures.

BACKGROUND At present, data about the cross-reactivity of Blomia spp. comes from studies made among different genera of mites, and no results have been published involving different species of the genus Blomia. OBJECTIVE The aim of this study was to find out the level of cross-reactivity between the two main species of Blomia causing allergy, and its implication in the diagnosis of Blomia sen...

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Analysis of the allergen expression of Blomia tropicalis and Blomia kulagini (Astigmata: Glycyphagidae) cultures.

Laboratory cultures of the mites Blomia tropicalis (van Bronswijk, Cock & Oshima) and Blomia kulagini (Zakhvatkin) were used to study the population dynamics of the mites and the kinetics of released allergens during the growth cycle. The analysis of extracts obtained after different incubation periods, by means of immunoblotting, and quantification of the major allergen Blo t 5, allowed defini...

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Genetic polymorphisms and allergenicity of Blo t 5 in a house dust mite allergic Filipino population.

BACKGROUND Blo t 5, a major allergen from the house dust mite Blomia tropicalis had been identified as an important trigger of allergic diseases. Effects of sequence variations to the IgE binding activity of Blo t 5 remain unclear. OBJECTIVE The study determined gene polymorphisms of Blo t 5, expressed the recombinant Blo t 5 allergen in E. coli system and determined its IgE reactivity in hou...

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Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae

Crude extracts of house dust mites are used clinically for diagnosis and immunotherapy of allergic diseases, including bronchial asthma, perennial rhinitis, and atopic dermatitis. However, crude extracts are complexes with non-allergenic antigens and lack effective concentrations of important allergens, resulting in several side effects. Dermatophagoides farinae (Hughes; Acari: Pyroglyphidae) i...

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ژورنال

عنوان ژورنال: Scientific Reports

سال: 2019

ISSN: 2045-2322

DOI: 10.1038/s41598-019-48688-y